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This work presents a method for extracellular and intracellular pH measurements in live cells based on a combination of the bead injection (BI) technique and fluorescence microscopy. For extracellular pH measurement, cells are grown on fluorescent beads, packed into a small column by a sequential injection instrument, and fluorescence intensity from the beads stained by the indicator is recorded by...
A hydrogen peroxide biosensor was fabricated by coating a sol-gel-peroxidase layer onto a Nafion-methylene green modified electrode. Immobilization of methylene green (MG) was attributed to the electrostatic force between MG + and the negatively charged sulfonic acid groups in Nafion polymer, whereas immobilization of horseradish peroxidase was attributed to the encapsulation function of the...
A simultaneous method for the determination of ethanol and glycerol in wines based on the coupling of pervaporation-chemical derivatisation-photometric detection for ethanol and biochemical derivatisation-fluorimetric detection for glycerol is proposed. After separation by pervaporation the ethanol is collected in a K 2 Cr 2 O 7 acceptor stream and the Cr 3+ formed...
In power stations, the cooling effluents are chlorinated to avoid excessive biofouling. Yet, this disinfecting treatment leads to the formation of halogenated by-products, mainly trihalomethanes. So, there is a need for precise and accurate methods that allow trace levels determination of these compounds. A system that combines purge-and-trap and gas chromatography (with an electron capture detector)...
The masking action of the dithiophosphoric acid diacylester (DDTP) for the determination of Bi by hydride generation absorption spectrometry (HGAAS) in the presence of high concentrations of copper was studied. Cu/Bi ratios up to 50x10 3 were found to be tolerable in the presence of 2x10 -3 M chelating solutions in 10% HCl, using 1.5% NaBH 4 as reductant. Good agreement...
A QSRR method was followed to relate the observed Kovats retention indexes of saturated alcohol compounds with their molecular connectivity indices by means of multilinear regression analysis and artificial neural networks technique. The alcohols included linear, branched with hydroxyl group on a primary, secondary, or tertiary carbon atom. At first, models were generated for six OV (Ohio Valley)...
A flow-injection system with a Chelite-S(R) cationic resin packed minicolumn is proposed for the determination of trace levels of mercury in agroindustrial samples by cold vapor atomic absorption spectrometry. Improved sensitivity and selectivity are attained since mercuric ions are on-line concentrated whereas other potential interferents are discarded. With on-line reductive elution procedure, concentrated...
A new approach to the determination of emulsifier OP (EOP, a nonionic surfactant) is proposed, based on the voltammetry of the EOP facilitating Ca 2+ transfer across the water/nitrobenzene (w/nb) interface. The peaks obtained with cyclic voltammetry at the w/nb interface correspond to the transfer of the coordination complex ion [Ca(EOP)] 2+ from the aqueous to the nonaqueous...
FT-Raman spectroscopy based on band intensity and band area measurements, was used for the quantitative determination of diazinon in pesticide formulations. Bands at 554, 604, 631, 1562 and 2971 cm -1 were used for calibration. Spectra were acquired by averaging 100 scans at a resolution of 4 cm -1 . Calibration curves were linear (correlation coefficients, 0.992-0.9992 and...
In atomic absorption spectrometric measurements calibration lines are measured daily. These lines are not always acceptable. They can, for instance, contain outliers, have a bad precision or can be curved. To evaluate the quality of those lines a method which gives a fast diagnosis is recommended. In this study the use of Kohonen neural networks was examined as an automated procedure to classify these...
A method for the preconcentration and speciation of chromium was developed. After formation of an anionic compound with ethylenediaminetetraacetic acid (CrY - ), Cr (VI) and Cr (III) are retained on a strong anionic phase (SAX) and controlled elution with 0.5 M NaCl permits their speciation. The retention and elution conditions were optimised, and interferences due to the presence of other...
Praseodymium forms a Pr(LMFX) 3 complex with lomefloxacin. In this paper, the absorption spectra of the complex has been investigated by applying conventional and derivative spectrophotometric methods. It was found that lomefloxacin could form a stable complex with praseodymium in the pH 6.5-8.5 media. The absorption intensity of the complex is 4.5-fold more than PrCl 3 . Using the...
A flow-through fluorescent sensor for the consecutive determination of Fe(III) and total iron is described. The reactive phase of the proposed sensor, which has a high affinity for complexed Fe(III), consists of pyoverdin immobilized on controlled pore glass (CPG) by covalent bonding. This pigment selectively reacts with Fe(III) decreasing its fluorescence emission. Total inorganic iron is determined...
Air transported samples of a mixture containing the analyte and a supporting electrolyte are consecutively propelled into a microconduit system through a titration cell, a coil and a detector cell. Different amounts of electrochemically generated titrant are obtained in the separate samples while they are passing through the titration cell. Various quantities of the titrant are achieved by changing...
The use of an amperometric biosensor for the salicylate determination in blood serum is described. The biosensor is based on salicylate hydroxylase (EC 1.14.13.1) electropolymerized onto a glassy carbon-working electrode with polypyrrole and glutaraldehyde, to improve the biosensor lifetime. The hexacyanoferrate (II) was also incorporated to work as a redox mediator to minimize possible interferences...
The principles of chemiluminescence and its applications as diagnostic tool are reviewed. After an introduction to the theoretical aspects of luminescence and energy transfer, the different classes of chemiluminogenic labels including luminol, acridinium compounds, coelenterazine and analogues, dioxetanes, systems based on peroxyoxalic acid and their derivatives are described emphasizing the molecules...
A novel chemiluminescence (CL) flow system for the determination of quinine is described. It is based on the direct chemiluminescence reaction of quinine and cobalt(III) in sulfuric acid medium. The unstable Co(III) was on-line electrogenerated by constant-current electrolysis. The chemiluminescence intensity was linear with a quinine concentration in the range of 0.1-100 μg ml -1 . The...
Proposed is a simple and reliable method for the dissolution of granite and the determination of 38 elements by inductively coupled plasma mass spectrometry. One hundred milligrams of sample are digested with 1 ml of HF and 0.5 ml of HNO 3 in screw top PTFE-lined stainless steel bombs at 190 o C for 12 h. Insoluble residues are dissolved using 8 ml of 40% HNO 3 (v/v) heated...
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